Cytotoxicity GROUPS OF COMPOUND AND IDENTIFICATION OF SECONDARY LEAF metabolites GAHARU Aquilaria malaccensis Lamk and Gyrinops versteegii (Gilg.) Domke AGAINST CANCER CELLS KOLON WiDr
ABSTRACT: Agarwood plants known as the famous name of woody plant producing resin in the heartwood and have good fragrant. The intensive cultivation of agarwood plants are coming from Aquilaria malaccensis Lamk dan Gyrinops versteegii(Gilg.) Domke. Those leaves have high antioxidant activity. The activity of high antioxidant have strong correlation with ability of leaves extract as anticancer. The purposes of this research are to determined the potential fraction and to identify the group of compound content from two species agarwood with the highest cytotoxicity on colon cancer cell lines WiDr. The specificity of potential extract was tested on Vero cells and the mechanism of cytotoxicity of potential extract was analyzed using flow cytometry methods. Agarwood leaves samples of A. malaccensis dan G. versteegii were collected from Agarwood Orchard in Klaten, Central Java. Samples were extracted using soxhletation method with 3 solvents such as chloroform, ethanol and aquadest. The cytotoxicity of each extract was analyzed using MTT assay on WiDr cells and Vero cells. The most potent extract was fractionated using VLC method with nonpolar to polar solvents. The most potent fraction was monitored using TLC method and detected using detector reagent to obtain the group of compound in fraction. The cell death mechanisms of the most potent fraction were analyzed using flow cytometryand double staining methods. The determination of IC50 value was calculated using probit analysis, whereas the data of the group compound and cell death were analyzed descriptively and qualitatively. The results showed that the chloroform extract of G.versteegii leaves and the fraction that eluted by n-hexane: chloroform (1:1) have the highest cytotoxicity value with 224,5 and 12,87 ���µg/mL, respectively. The group of compound were potentially as anticancer including terpenoids, flavonoids, phenolics and tannins. The selectivity assay on Vero and WiDr cells showed the potent fraction that eluted by n-hexane: chloroform (1:1) as the highest selectivity with index value 11,2. The cytotocixity of potential extract fraction induced necrosis and apoptosis on cancer cell line WiDr, there by potentially developed as an colon cancer therapy
ABSTRACT: Agarwood plants known as the famous name of woody plant producing resin in the heartwood and have good fragrant. The intensive cultivation of agarwood plants are coming from Aquilaria malaccensis Lamk dan Gyrinops versteegii(Gilg.) Domke. Those leaves have high antioxidant activity. The activity of high antioxidant have strong correlation with ability of leaves extract as anticancer. The purposes of this research are to determined the potential fraction and to identify the group of compound content from two species agarwood with the highest cytotoxicity on colon cancer cell lines WiDr. The specificity of potential extract was tested on Vero cells and the mechanism of cytotoxicity of potential extract was analyzed using flow cytometry methods. Agarwood leaves samples of A. malaccensis dan G. versteegii were collected from Agarwood Orchard in Klaten, Central Java. Samples were extracted using soxhletation method with 3 solvents such as chloroform, ethanol and aquadest. The cytotoxicity of each extract was analyzed using MTT assay on WiDr cells and Vero cells. The most potent extract was fractionated using VLC method with nonpolar to polar solvents. The most potent fraction was monitored using TLC method and detected using detector reagent to obtain the group of compound in fraction. The cell death mechanisms of the most potent fraction were analyzed using flow cytometryand double staining methods. The determination of IC50 value was calculated using probit analysis, whereas the data of the group compound and cell death were analyzed descriptively and qualitatively. The results showed that the chloroform extract of G.versteegii leaves and the fraction that eluted by n-hexane: chloroform (1:1) have the highest cytotoxicity value with 224,5 and 12,87 ���µg/mL, respectively. The group of compound were potentially as anticancer including terpenoids, flavonoids, phenolics and tannins. The selectivity assay on Vero and WiDr cells showed the potent fraction that eluted by n-hexane: chloroform (1:1) as the highest selectivity with index value 11,2. The cytotocixity of potential extract fraction induced necrosis and apoptosis on cancer cell line WiDr, there by potentially developed as an colon cancer therapy
