CXCL12 mRNA EXPRESSION CONNECTING WITH STATUS ON METASTASIS infiltrating ductal carcinoma
ABSTRACT: Carcinoma of the breast is the most common carcinoma in women in the world and Indonesia. The factors that lead to death are due to the presence of metastasis and recurrency. SDF-1/CXCL12, a chemokine that is chemoattractant, is known to have important role in angiogenesis, carcinogenesis and related to the metastasis of carcinoma of the breast as well as it can be used as a prognostic factor. The role of SDF- 1/CXCL12 mRNA expression as a prognostic marker and metastatic factor in carcinoma of the breast is still controversial. This cross-sectional study is to investigate the mRNA expression of CXCL12 in carcinoma of the breast tissue related to the metastatic or non metastatic status. Forty six paraffin blocks from tumor tissue with histopathological diagnosis of invasive ductal carcinoma, NST, grade III with metastatic and non metastatic status, was used in this research. RNA extraction was performed from the parrafin blocks, subsequently reverse transcriptase PCR with random primer was performed to obtain cDNA. The mRNA expression of CXCL12 was quantified with quantitative real time PCR method, involving house-keeping gene and normal breast tissue were used for normalisation. The results showed that the mRNA expression of CXCL12 was expressed both in metastatic and non metastatic breast carcinoma. The mRNA expression of CXCL12 in non metastatic carcinoma was higher than those in metastatic carcinoma, however it was not significant statistically. There is no significant difference of mRNA expression of CXCL12 in non metastatic and metastatic carcinoma of the breast. The expression level of CXCL12 in non metastatic carcinoma is relatively higher compared to those with metastasis. Therefore this gene may be considered to be protective marker for metastasis by lowering chemotaxis.
