ANTICANCER ACTIVITY LEAF EXTRACT AIR SINGKONG (Manihot esculenta Crantz.) BOILED AND RAW ON CANCER CELLS IN VITRO BY KOLON WIDR
ABSTRACT: Background: Cancer is a global health problem with an increasing number of sufferer each year. While the three main cancer is most often detected are lung cancer, breast cancer, and colon cancer. Consumption of fruits and vegetables is known to reduce the risk of colon cancer, though it is still being debated. Cassava leaves rich in essential nutrients for the body; such as carbohydrates, vitamin A, vitamin C, iron, calcium, and phosphorus. Cassava leaves themselves are consumed as a vegetable. It is also used by the people of Indonesia to treat various diseases, including as a food ingredient that is believed to function as an anti-cancer. However, not much research on the anti-cancer activity of cassava leaves, especially in colon cancer. Objective: The aim from this study is to investigated the anti-cancer activity of the leaves of cassava (Manihot esculenta Crantz) raw or boiled on the against of colon cancer cells WiDr. Methods of study: Effect of crude aqueous extract of raw cassava leaves and boiled cassava leaves tested in colon cancer cells WiDr. Anti-cancer testing using the MTT method with parameters such as the percentage of inhibition. Results: raw cassava leaf water extract gave IC50 of 63.1 mg / ml. While the water extract of boiled cassava leaves gave IC50 of 79.4 mg / ml. However, there is no difference ability of anticancer activity of raw and boiled cassava leaves on the inhibition of colon cancer cells. Conclusion: Cassava leaves have anticancer activity against colon cancer cells WiDr. In addition, the boiling process does not reduce the anticancer activity on cassava leaves.
