Cytotoxicity LEAF EXTRACT FROM MEKAI Aspergillus fumigatus (Albertisia papuana Becc.) AGAINST BREAST CANCER CELLS T47D and MCF-7
ABSTRACT: The previous studies showed that the secondary metabolism of Albertisia papuana Becc. root have cytotoxicity on breast cancer. The A. papuana root cytotoxicity on breast cancer could not only be produced by plant secondary metabolism, but may be also by secondary metabolism of endophytes. Aspergillus fumigatus is one of endophytes that have anticancer agent. Endophytes can be distributed dynamically in whole of plant organ, one of them is leave. Therefore the objective of this studies were to know the presence of A. fumigatus in A. papuana leaves, and the cytotoxicity of their secondary metabolism on breast cancer cells. The sample of A. papuana were collected from Botanical Zoo of Bogor, while T47D and MCF-7 cell lines were obtained from Faculty of Medicine, UGM. Isolation of endophytes was done by growing leaves extract on water agar 2% medium. Secondary metabolism was extracted from fermented broth of A. fumigatus in ethyl acetat and n-butanol. The cytotoxicity was performed by MTT (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The result showed that A. fumigatus was associated with A. papuana leaves. Ethyl acetat extract from fermented both on T47D and MCF-7 cell lines had better (IC50. 0,044 mg/ml and 0,049 mg/ml) than n-butanol (IC50. 0,078 mg/ml and 0,117 mg/ml). The morfological character of A. fumigatus were greenish in colony colour and it’s white marginal colour. The hyphal was hyalin, aseptat, branched and had a foot cell. Conidia shapes were globose dan subglobose. The diameter of conidia was 2-3 μm. The length of conidiophore was 80 μm x 2,5 μm. Phialida shape was amphuliform. The lenght of phialida was 5 μm. The diameter vesicle was 15 μm x 18 μm, and have not metulae and sclerotia.
ABSTRACT: The previous studies showed that the secondary metabolism of Albertisia papuana Becc. root have cytotoxicity on breast cancer. The A. papuana root cytotoxicity on breast cancer could not only be produced by plant secondary metabolism, but may be also by secondary metabolism of endophytes. Aspergillus fumigatus is one of endophytes that have anticancer agent. Endophytes can be distributed dynamically in whole of plant organ, one of them is leave. Therefore the objective of this studies were to know the presence of A. fumigatus in A. papuana leaves, and the cytotoxicity of their secondary metabolism on breast cancer cells. The sample of A. papuana were collected from Botanical Zoo of Bogor, while T47D and MCF-7 cell lines were obtained from Faculty of Medicine, UGM. Isolation of endophytes was done by growing leaves extract on water agar 2% medium. Secondary metabolism was extracted from fermented broth of A. fumigatus in ethyl acetat and n-butanol. The cytotoxicity was performed by MTT (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The result showed that A. fumigatus was associated with A. papuana leaves. Ethyl acetat extract from fermented both on T47D and MCF-7 cell lines had better (IC50. 0,044 mg/ml and 0,049 mg/ml) than n-butanol (IC50. 0,078 mg/ml and 0,117 mg/ml). The morfological character of A. fumigatus were greenish in colony colour and it’s white marginal colour. The hyphal was hyalin, aseptat, branched and had a foot cell. Conidia shapes were globose dan subglobose. The diameter of conidia was 2-3 μm. The length of conidiophore was 80 μm x 2,5 μm. Phialida shape was amphuliform. The lenght of phialida was 5 μm. The diameter vesicle was 15 μm x 18 μm, and have not metulae and sclerotia.
