ANALYSIS micronucleus cytogenetic buccal mucosa CANCER PATIENTS IN THE HEAD AND NECK AREA AFTER RADIATION THERAPY (Studies in General Hospital Dr. Sardjito, Yogyakarta)
ABSTRACT: Radiation therapy plays an important role in the treatment ofhead and neck cancer. The purpose of this therapy is to kill cancer cells as many as possible by increasing ionization in the involved area. The oral of head and neck cancer patients has a high risk to radiation therapy because this therapy could damage the normal cell. The damage that might be happen such as the formation of micronucleus in the basal layer of epithelial buccal mucosa. The aim of this study was to determine the influence of radiation therapy in head and neck cancer patients to frequency of micronucleus. This study was conducted on 20 patients of head and neck cancer at RSUP Dr. Sardjito, Yogyakarta. They were divided into 2 groups, group I was performed by 10 patients who have not receivedradiation therapy and group II, as many as 10 patients receivedradiation therapy10 Gy within one week. The buccal ephitelial cells werecollected with a cytobrush and stained with Feulgen Fast-Green. The frequency of micronucleus was counted per 1000 cells using light microscope. The data were analyzed using independent t-test and one-way ANOVA. The results showed a significant difference inmicronucleus frequency between 2 groups. It is concluded that there was a significant increase in micronucleus frequency of ephitelial buccal mucosa cellsin head and neck cancer patients after receiving radiation therapy compared to micronucleus frequency before receiving radiation therapy. The increase of micronucleus frequency occured along with increased of radiation dose.
