SYNTHESIS AND cytotoxicity assay 3 ', 4'-dimethoxy-7-HIDROKSIISOFLAVON AGAINST CERVICAL CANCER CELLS (HeLa CELL LINE)
ABSTRACT: Synthesis and anticancer was to in-vitro test of 3',4'-dimethoxy-7-hidroxyisoflavone compunds from recorcinol and 3,4-dimethoxybenzil carboxilate acid have been carried out. The objective of this study was to synthesize 3',4'-dimethoxy-7-hidroxyisoflavone using two pot procedure and one pot procedure, followed by comparing the effectiveness. Synthesis have been done with conventional method using two pot procedure and one pot procedure. Two pot procedure was done within isolation 3,4-dimethoxybenzil-2',4'-dihidroxyphenil ketone as an intermediate. Resorcinol and 3,4-dimethoxybenzil carboxilate acid was solved in BF3.Et2O followed by heating at 70 degree C for two hours. The mixture was poured to cold solution of natrium acetate 12% then the precipitate was pured. That intermediate was 3,4-dimethoxybenzil-2',4'-dihydroxiphenil ketone. It was used for next step synthesis. Addition of one carbone was done with DMF and cyclication to be isoflavone was done with DMF/POCl3 mixture at room temperature for four hours. One pot procedure have been done with identic chemical compotition and reaction duration without intermediate purification. Confirmation of molecules structure was identified by FTIR spectrophotometer, GC-MS, 1H-NMR and 13C-NMR. Product 3',4'-dimethoxy-7-hidroxyisoflavone activity was tested in cervical cancer (HeLa Cell Line) with MTT assay method. Based on the research 3',4'-dimethoxy-7-hidroxyisoflavone was obtained as white solid in 70,60% yield. Synthesis of 3',4'-dimethoxy-7-hidroxyisoflavone was with two pot procedure gave white-brown solid product with 51,54% yield and 34,60% is given by one pot procedure. Anticancer activity test againts HeLa cell line gave inhibitor concentration number (IC50) 60,34 microgram/mL. The result was showed that compund was inactive as inhibitor HeLa cell line.
