Cytotoxicity FRACTION AND METHANOL chloroform extract vegetative organ of Dioscorea bulbifera L. AGAINST BREAST CANCER CELLS T47D
ABSTRACT: Dioscorea bulbifera is a plant that is commonly used as ingredient of traditional medicine. Some researches have reported that D. bulbifera have anticancer potential. Breast cancer is the second leading cause of cancer deaths in women. Therefore, the objective of the studies were to elucidate cytotoxicity of chloroform and methanol extract fractions of D. bulbifera vegetative organ on T47D breast cancer cell line. The vegetative organ of D. bulbifera were extracted gradually using chloroform and methanol. All extracts were tested on breast cancer cells (T47D) by MTT Assay method. The most toxic extract was fractioned by vacuum liquid chromatography (VLC) and followed by thin layer chromatography (TLC). The similar TLC profiles were combined. The toxicity of combined fraction were tested on the T47D cells by the MTT Assay. The most toxic fraction was isolated by Preparative TLC. The toxicity of isolats were tested on the T47D cells by the MTT Assay. The toxicity of extract and fraction potential were tested on the Vero cells by MTT Assay. The most toxic fraction was also analyzed using TLC followed by the application of various detection reagents for identification of the active compound. Tes cytotoxicity by MTT Assay method showed that the chloroform extract of the D. bulbifera leaves had the highest cytotoxic effect on T47D cells (IC50 115.63±86.01 µg/mL). Cytotoxicity test showed that the combined fraction of chloroform extract fractions F5 (ethylacetat:methanol 20:1, 10:1 and 5:1) and F6 (ethylacetat:methanol 3:1 and methanol 100%) had the high cytotoxic (IC50 = 14.55±8.62 and 7.12±4.43 µg/mL). Preparative TLC mixtures of F5 and F6 (FC) fractions produced 5 isolates. The top fraction (FC1) has the highest cytotoxic activity (IC50 122.27±5.26 µg/mL). However, this activity still lower than Doxorubicin (IC50 0.04±0.02 µg/mL). Fraction potential was not toxic against Vero cells with IS>10. The active compound in those fraction was terpenoid and alkaloid.
ABSTRACT: Dioscorea bulbifera is a plant that is commonly used as ingredient of traditional medicine. Some researches have reported that D. bulbifera have anticancer potential. Breast cancer is the second leading cause of cancer deaths in women. Therefore, the objective of the studies were to elucidate cytotoxicity of chloroform and methanol extract fractions of D. bulbifera vegetative organ on T47D breast cancer cell line. The vegetative organ of D. bulbifera were extracted gradually using chloroform and methanol. All extracts were tested on breast cancer cells (T47D) by MTT Assay method. The most toxic extract was fractioned by vacuum liquid chromatography (VLC) and followed by thin layer chromatography (TLC). The similar TLC profiles were combined. The toxicity of combined fraction were tested on the T47D cells by the MTT Assay. The most toxic fraction was isolated by Preparative TLC. The toxicity of isolats were tested on the T47D cells by the MTT Assay. The toxicity of extract and fraction potential were tested on the Vero cells by MTT Assay. The most toxic fraction was also analyzed using TLC followed by the application of various detection reagents for identification of the active compound. Tes cytotoxicity by MTT Assay method showed that the chloroform extract of the D. bulbifera leaves had the highest cytotoxic effect on T47D cells (IC50 115.63±86.01 µg/mL). Cytotoxicity test showed that the combined fraction of chloroform extract fractions F5 (ethylacetat:methanol 20:1, 10:1 and 5:1) and F6 (ethylacetat:methanol 3:1 and methanol 100%) had the high cytotoxic (IC50 = 14.55±8.62 and 7.12±4.43 µg/mL). Preparative TLC mixtures of F5 and F6 (FC) fractions produced 5 isolates. The top fraction (FC1) has the highest cytotoxic activity (IC50 122.27±5.26 µg/mL). However, this activity still lower than Doxorubicin (IC50 0.04±0.02 µg/mL). Fraction potential was not toxic against Vero cells with IS>10. The active compound in those fraction was terpenoid and alkaloid.
