EFFECT OF PLANT EXTRACTS ethanolic ANT NESTS (Myrmecodia tuberosa) BARRIERS TO GROWTH AND MIGRATION AND INVASIVE CANCER CELLS INDUCED APOPTOSIS (in vitro study)
ABSTRACT: Ant nest (Myrmecodia tuberosa) is a traditional plant that is known to have anticancer compound which has been examined in vitro and in vivo. Active compound of ant nest is reported to inhibit the growth of human cancer cell types. In the present study, ant nest powder was incubated in ethanol 80% using maceration method for evaluating anti-cancer activity on KB cells. The research objective was to evaluate the effect of ethanolic extracts of ant nest against growth inhibition induction of apoptosis and migration in invasif cancer cell. Pure experimental laboratory with a post-test only control group design was confirmed in this study. Methyl tetrazolium test (MTT assay) was conducted to determine the growth inhibition of KB cells, and staining analysis with double-staining was performed to evaluate the induction of apoptosis of KB cells. Scratch wound healing technique was carried-out to observe the migration cells. Statistical analysis was done with SPSS 16.0 program, and data were analyzed using the Kruskal-Wallis test followed by Mann-Whitney U test with a significance level of 95%. The results revealed concentration of 62.5, 125, 250, 500 and 1000 mikrogram/ml was markedly increased the KB cell growth inhibition. The percentage average of growth inhibition was 35.5%, 39.5%, 58.3%, 68.5% and 84.2% respectively, compared to the negative control. Apoptosis of KB cells was significantly induced after incubation of various concentration of ethanolic extract of ant nest for 24 hours. Increased in the percentage of apoptotic KB cells also occurred at concentration 62.5 to 1000 mikrogram /ml. Furthermore, cell migration was markedly increased the inhibitory ability of 4T1 cells treated with various concentration of extract. In conclusion, ethanolic extract of ant nest plant had the inhibition effect on cell growth, induction of apoptosis of KB cells and migration of 4T1 cells.
