EFFECTIVENESS EXTRACT purwaceng water (Pimpinella alpina) IN LUNG CANCER CELLS anti-proliferation activities A549
ABSTRACT: Background: Lung cancer is an non communicable disease which has high incidence and mortality rate among another cancer around the world. Conventional chemotherapy has many high undesirable side effect which make living cells death. Alternative chemotherapy agents with low side effect is needed to inhibit proliferation by tumor suppressor genes pathway such as p53 and Rb. Herbal plant Pimpinella alpina has an active compound Coumarin and alkaloid which potentially to inhibit proliferation lung cancer cell line A549. Objective: to find out antiproliferative effect and p53 and Rb gene expression pathway of water extract Pimpinella alpina towards lung cancer cell line A549. Method: The study is purely experimental ,post test only with control group design which use sample lung cancer cell line A549 and water extract Pimpinella alpina (1-6000 µg/ml)and positive control Cisplatin (1-500 µg/ml).Cytostatic test of lung cancer cell line A549 has been incubated 24 hours using test solvent and positive control then stained using MTT to show percentage of cell living. Antiproliferation test is incubated in various time 24, 48, and 72 hours then stained by MTT to show cell living percentage of lung cancer cell line A549. Gen expression test on growth inhibition pathway using immunohistochemistry staining antibody p53 and Rb. Result: Water extract Pimpinella alpina has an antiproliferative effect with IC50 2.605,785 μg/mL could inhibit cell growth of lung cancer cell line A549 on concentration 550 µg/ml towards 24 , 48, and 72 hours significantly. Antiproliferative effect of water extract Pimpinella alpina is shown on concentration 2250 μg/mL by the increasing of p53 expression in the amount of 3,57% but it has not proven yet the effect of water extract of Pimpinella alpina toward Rb expression to inhibit lung cancer cell line A549 proliferation Conclusion: Pimpinella alpina has an antiproliferative effect by inhibit the cell growth on 24, 48, and 72 hours and by the increasing on p53 expression but it has not proven yet the effect of water extract of Pimpinella alpina toward Rb expression to inhibit lung cancer cell line A549 proliferation. Keywords: Lung cancer, Pimpinella alpina, Cytostatic, Antiproliferative
