Effects methanolic extract Moss Heart (marchantia polymorpha L.) Against Heat Shock Protein 70 Expression Cell Culture HeLa
ABSTRACT: Liverwort (Marchantia polymorpha L) is one of plant that has high active compound such as flavonoid, terpenoid, fenolik that have potential as anticancer. This research aimed to determine the effect of liverwort extract to HSP 70 expression, viability, apotosis induction and nitrit oxide concentration. Liverworts leave were extracted using methanol solvent. HeLa cell line is used as model of treatment. Cell were incubated 48 hours in RPMI-1640 and then treated with eight concentration of liverworts extract such negative control, positive control (doksorubsin 7 ug/mL) and 50, 100, 150, 200, 250, 300 ug/mL extract between IC50 concentration. Test of active compound of liverwort using KLT method, viability Hela cell using WST-1 method, apoptosis cell using fluoresen microscop. Nitrit oxide measurement using griess assay method. Heat shock protein 70 were detected using western blot method. Analyz data used kruskawallis continued Gomez Howell and Bronfenni. Liverwort revelead the presence of flavonoid, terpenoid and phenolic. Cytotoxity assay showed hat the growth inhibition of HeLa in concentration 186 ug/mL and potential to induced HeLa cell line apoptosis. Concentration of nitrit oxide and quantification protein 70kDa of HeLa cell line were decreased after treatment of liverwort methanolic extract.
