TEST POTENTIAL ANTICANCER ISOLATE OF CULTURE MEDIA FUNGI Aspergillus fumigatus endophyte strains KARSV04 AGAINST BREAST CANCER CELLS
ABSTRACT: ABSTRACT Doxorubicin is a breast cancer chemotherapy agent that is often used in the treatment, causing resistance and side effects, therefore the necessary efforts to improve the effectiveness and decrease side effects. Ethyl acetate extract and isolates 4 from culture medium endophytic fungi Aspergillus sp. isolated from plant stems Piper crocatum Ruiz and Pav. (BS 1) have known showed cytotoxic activity against T47D cell line and does not affect vero cell line. The aim of this study is to assess potential anticancer isolate ethyl acetate extract from the culture medium endophytic fungi Aspergillus fumigatus strain KARSV04 (BS 1) and when combined with doxorubicin on T47D and MCF-7 cell line also tested selectivity against normal cells Vero. Secondary metabolite production was carried out through fermentation using the PDB as medium for 10 days and the medium was extracted using liquid-liquid extraction with ethyl acetate solvent. Fractionation and isolation was done using TLC preparative. In cytotoxicity assay, T47D, MCF-7 and vero cells were cultured in the presence of pure isolate, doxorubicin, and a combination of both using MTT assay with IC50 values and Selectivity Index (SI) as a parameters to evaluate effectivity of anticancer. The percentage of cell viability and CI (Combination Index) as a parameter of effectivity combination. Isolate and doxorubicin has IC50 values 167,15 µg/mL and 0,129 µg/mL against T47D cell line. Combinations of isolates and doxorubicin all doses showed antagonistic effect on T47D cells, except combinations isolates dose 25 µg/mL and 0,25 µg/mL doxorubicin which have an additive effect. IC50 value of isolate and doxorubicin against MCF-7 cell line are 90,64 µg/mL and 0,363 µg/mL. Further, combinations among isolates and doxorubicin at all doses showed synergistic effects on MCF-7 cell line.
