IDENTIFICATION OF TYPE ANTIOXIDANT COMPOUNDS AND LEAVES GAHARU cytotoxicity (Gyrinops versteegii (Gilg.) Domke) ON CELL Cervical cancer (HeLa)
ABSTRACT: A number of tradition came to dominate the practice of herbal medicine in the Western world at the end of the twentieth century. In developed countries, the practice of herbal medicine could be connected with the trend favourable to the socalled a sustainable development and to reducing public health cost. Recently, several part of agarwood have been extensively used to treat diarrhoea, dysentery, antiinflamation and skin diseases. The scientific studies of antioxidant and anticancer activity of agarwood leaf Gyrinops versteegi relatively very few. The objective of this study was to identify bioactive group of compound and to know the difference of antioxidant activity between young and old leave and also to analyze the extracts which have cytotoxic effects on cervical cancer cells (HeLa). The young and old Gyrinops versteegii leaves collected from Bogor botanical garden was dried and extracted consecutively using three solvents chloroform, methanol, and water by soxhletation method. The test of antioxidant activity was conducted using DPPH and cytotoxic test using MTT assay method. The antioxidant potential extract was fractionated by VLC method and the identification group of compounds using TLC followed by the detection by detector reagent. The value of IC50 extract was determined by probit analysis. ANOVA was used to analyze the difference among samples followed by DMRT to locate the difference, whereas kruskal wallis to distribution of abnormal data and followed by games howel. The result showed that the methanol extract of old agarwood leaf and the combined fraction II (methanol: ethyl acetate = 1: 1 and methanol: ethyl acetate = 5: 1) has the high antioxidant activity with IC50 value of 11,659 microgram/ml and12,958 microgram/ml respectively. The bioactive group of compound which was efficacious as antioxidant were phenols, flavonoids, and tannin. The total content of phenols in leaves was lower in elderly than the young leaves. In contrast, total flavonoids old leaves were higher than the young leaves. The chloroform extract of young leaves had the highest cytotoxic activity with IC50 value of 11,37 microgram/ml. Bioactivity of agarwood leaves as antioxidant and anticancer towards HeLa cell line affected by the developmental stages of the leaves. Methanol extract of gaharu old leaves have the higehst antioxidant activity, whereas chloroform extract of gaharu young leaves have the highest anticancer activity.
