Induction of Apoptosis Ethyl Acetate Extracts Isolates Sponge-Associated Fungi against Cervical Cancer Cell Line (HeLa)
ABSTRACT: Cervical cancer is the second leading cause of death in women today and is the most common cancer among women. Chemoterapy is one of cancer treatments but it will cause multidrug resistance. Recently, natural products from marine life especially Sponge-Associated Fungi have been extensively used to treat cancer. Last researches had isolated compound which had anticancer effect. Therefore the objective of these studies were to analyze the cytotoxicity of ethyl acetate of KU4 extract from KU4 medium and combination of mycelium-medium and to study the apoptotic effect on cervical cancer cell line (HeLa). KU4 culture in broth media and the combination were macerated and extracted using ethyl acetat, then determined IC50 by MTT Assay. Results of IC50 value were used to induce apoptotic on cervical cancer cell line (HeLa) that determined by double staining Acridine Orange/Ethidium Bromide. Datum obtained were analyzed qualitatively and quantitatively. The results showed that IC50 of extract media and combination were 158,13 mikrogram/mL and 283,95 mikrogram/mL respectively. Both of two extracts could induce apoptotic on HeLa cancer cell line.
