Dichloromethane extract cytotoxicity Parmotrema tinctorium Delise against MCF7 Breast Cancer Cells
ABSTRACT: Breast cancer is a disease which considers as first rank cancer case in Indonesia. Research to obtain anticancer drugs with minimum side effect is still very necessary. One of natural source material that are abundant and have the potential to produce natural anticancer compound is lichen. Parmotrema tinctorium is a lichen which has a wide habitat distribution in Indonesia. The previous study showed that dichloromethane extract of P. tinctorium has a potential as natural anticancer drug. The purpose of this study was to determine the cytotoxicity of dichloromethane extract of P. tinctorium against MCF7 breast cancer cell cultures and to determine the group of compounds which contain in the extract. P. tinctorium was collected from Plawangan hill, Kaliurang, Yogyakarta in November 2014. Sample was dried in oven and powdered using blender. Sample extraction was done by maceration with three solvent mixture of dichloromethane and methanol with consecutive ratio of 1:3, 1:1 and 3:1. Determination of which extract to be tested on MCF7 cell line was done based on TLC profile. The extract derived from dichloromethane:methanol in ratio of 3:1 was selected since this contained the most number of spots in TLC. This extracts was tested on MCF7 cultures by MTT assay at concentrations of 2.93 to 1500 mcg/ml. The content of group of compounds from selected extract was performed by TLC and followed by spraying with several dye reagents: Dragendorf, ammonium steam and cerium (IV) sulfate. The results of MTT assay test showed that the extract has IC50 value of 237.75 mcg/ml. The group of compounds contained in the selected extract were alkaloids, phenolics and terpenoids.
