Expression of COX-2 in the liver WHITE RATS [SPRAQUE Dawley] DMBA-induced COMPOUNDS AND EXPOSURE 1.2 EPOXY-3- [3- (3,4-DIMETOKSIFENIL) 4H-1-benzopyran-4-ON] PROPANE
ABSTRACT: Background: Cancer is the leading cause of death in the world . Potential natural materials to be developed as anticancer compounds includes flavonoids group. Isoflavones are one of the flavonoid compounds. Isoflavones have various pharmacological activities such as antioxidant, anti-inflammatory, antimutagenic, and anticancer. This study was conducted to determine the expression of cox - 2 in the liver of Spraque Dawley rats exposed to1,2 epoxy - 3 - [ 3 - ( 3,4 - dimetoksifenil ) 4H - 1 - benzopyran - 4-one ] propane ( EPI ) and the induction of DMBA. Expression of COX - 2 is one of the signs of inflammation and is a major contributing factor in carcinogenesis. Objective: To determine the expression of COX - 2 Spraque -Dawley rats liver cells that have been induced by DMBA and EPI. Methods: Thirty- six rats were divided into 6 groups. Every 3 days was conducted clinical observation namely body weight and viability on the test animals. Rats were given 20mg/kg DMBA body weight dissolved in corn oil and doses of 1 mg, 2 mg and 4 mg / kg BW of EPI compounds. DMBA and corn oil injection were conducted twice a week for 5 weeks. Administration of food and drink equated for all groups namely AD II standard feed and ad libitum aqua drinking water. Observation conducted for 15 weeks, after which the rats were sacrificed and sent to the histology preparations for the manufacture of paraffin blocks and IHC coloring with monoclonal antibody anti- COX-2 rats. Histological observation of the liver of rats aimed to see the expression of COX-2. Calculation of COX-2 expressions was measured using a light microscope at 5 visual fields with 40x magnification. COX-2 expression was characterized by the brown color in the cytoplasm cell. The average yield from each group was analyzed by ANOVA parametric statistical methods. Results: Normal group with the corn oil group, 1 mg EPI, 2 mg and 4 mg did not differ significantly (p > 0.05). DMBA group expressed most COX - 2. Rat liver of EPI group expressed COX - 2 lower than the DMBA group (p < 0.05). Conclusion: Administration of EPI compounds was able to reduce the expression of COX - 2 in liver cells Spraque -Dawley rats that had received exposure to DMBA induction
ABSTRACT: Background: Cancer is the leading cause of death in the world . Potential natural materials to be developed as anticancer compounds includes flavonoids group. Isoflavones are one of the flavonoid compounds. Isoflavones have various pharmacological activities such as antioxidant, anti-inflammatory, antimutagenic, and anticancer. This study was conducted to determine the expression of cox - 2 in the liver of Spraque Dawley rats exposed to1,2 epoxy - 3 - [ 3 - ( 3,4 - dimetoksifenil ) 4H - 1 - benzopyran - 4-one ] propane ( EPI ) and the induction of DMBA. Expression of COX - 2 is one of the signs of inflammation and is a major contributing factor in carcinogenesis. Objective: To determine the expression of COX - 2 Spraque -Dawley rats liver cells that have been induced by DMBA and EPI. Methods: Thirty- six rats were divided into 6 groups. Every 3 days was conducted clinical observation namely body weight and viability on the test animals. Rats were given 20mg/kg DMBA body weight dissolved in corn oil and doses of 1 mg, 2 mg and 4 mg / kg BW of EPI compounds. DMBA and corn oil injection were conducted twice a week for 5 weeks. Administration of food and drink equated for all groups namely AD II standard feed and ad libitum aqua drinking water. Observation conducted for 15 weeks, after which the rats were sacrificed and sent to the histology preparations for the manufacture of paraffin blocks and IHC coloring with monoclonal antibody anti- COX-2 rats. Histological observation of the liver of rats aimed to see the expression of COX-2. Calculation of COX-2 expressions was measured using a light microscope at 5 visual fields with 40x magnification. COX-2 expression was characterized by the brown color in the cytoplasm cell. The average yield from each group was analyzed by ANOVA parametric statistical methods. Results: Normal group with the corn oil group, 1 mg EPI, 2 mg and 4 mg did not differ significantly (p > 0.05). DMBA group expressed most COX - 2. Rat liver of EPI group expressed COX - 2 lower than the DMBA group (p < 0.05). Conclusion: Administration of EPI compounds was able to reduce the expression of COX - 2 in liver cells Spraque -Dawley rats that had received exposure to DMBA induction
