MRNA expression of Caspase 3 Breast Cancer Cells T47D (Human Breast Cancer Cell Line) Induced by Leaf Extract Kaffir Lime (Citrus hystrix)
ABSTRACT: Kaffir lime (Citrus hystrix Dc.) is native indigenous species in Southeast Asia contains flavonoids, coumarins and limonoids were classified into chemoprevention compounds. Chemoprevention is a compound that can be used to hinder, delay, or induce apoptosis in cancer. Flavonoids from ethyl acetate extracts can induce apoptosis by activating caspase 8. Caspase 8 cleave BID (Bcl-2 family) and activates cytochrome c. Cytochrome c with Apaf-1 to form CARD (Caspase Recruitment Domain). CARD will merge to form apoptosome then bind and activate pro-caspase 9 into caspase 9. Caspase 9 activate caspase 3 and leads to apoptosis. This study aimed to examine the expression of caspase-3 in T47D cells after treated with ethyl acetate and chloroform extracts of leaves of kaffir lime (Citrus hystrix Dc.). Caspase 3 expression seen with the Two-Step Reverse Transcriptase PCR (RT-PCR) and PCR performed using specific primers and then the results were visualized using UV transiluminator. he results showed that five samples of doxorubicin treatment, DMSO, control cells, ethyl acetate and chloroform. Only ethyl acetate and doxorubicin can expressing caspase 3. In the kaffir lime leaf extract with ethyl acetate contains flavonoids that can induce apoptosis.
ABSTRACT: Kaffir lime (Citrus hystrix Dc.) is native indigenous species in Southeast Asia contains flavonoids, coumarins and limonoids were classified into chemoprevention compounds. Chemoprevention is a compound that can be used to hinder, delay, or induce apoptosis in cancer. Flavonoids from ethyl acetate extracts can induce apoptosis by activating caspase 8. Caspase 8 cleave BID (Bcl-2 family) and activates cytochrome c. Cytochrome c with Apaf-1 to form CARD (Caspase Recruitment Domain). CARD will merge to form apoptosome then bind and activate pro-caspase 9 into caspase 9. Caspase 9 activate caspase 3 and leads to apoptosis. This study aimed to examine the expression of caspase-3 in T47D cells after treated with ethyl acetate and chloroform extracts of leaves of kaffir lime (Citrus hystrix Dc.). Caspase 3 expression seen with the Two-Step Reverse Transcriptase PCR (RT-PCR) and PCR performed using specific primers and then the results were visualized using UV transiluminator. he results showed that five samples of doxorubicin treatment, DMSO, control cells, ethyl acetate and chloroform. Only ethyl acetate and doxorubicin can expressing caspase 3. In the kaffir lime leaf extract with ethyl acetate contains flavonoids that can induce apoptosis.
