Relationships number of colonies of Candida sp. Saliva-dose radiotherapy in patients with head and neck cancer (Study at Dr. Sardjito Hospital YOGYAKARTA)
ABSTRACT: Head and neck cancer has 2.8% of all malignancy cases that frequently occur in the world. The treatment includes radiotherapy, chemotherapy, combination chemotherapy and radiotherapy-surgery. Radiotherapy is aimed to disrupt the reproduction of malignant cells. Radiation may result in immediate and permanent complications, such as mucositis, hyposalivation, and xerostomia resulting oral hygiene becomes worse may cause imbalance of oral microba. The purpose of this study was to determine correlation between the number volume salivary colonies of Candida sp. and dose radiotherapy of head and neck cancer patients. This research is an observational cross-sectional study, subject was selected using consecutive sampling. Samples were brought to the Microbiology Laboratory of the Faculty to be cultured. Cultivation of Candida sp. by used dilution 1: 104 CFU / ml in Sabouraud dextrose agar medium and incubated for 48 hours at a temperature of 370C. After 48 hours the number of colonies were counted. The data obtained from this study were analyzed by using the Shapiro-Wilk normality, followed by Spearman correlation test. The test results showed the significant value of 0.000 (p<0,05) with r = 0.656. The conclusion is there a strong correlation between the number of colonies of Candida sp. the radiation dose received by the patient's head and neck cancer in the department of Dr. Sardjito hospital, the higher dose of radiotherapy received, the more colonies number colonies of Candida sp. in patients saliva.
ABSTRACT: Head and neck cancer has 2.8% of all malignancy cases that frequently occur in the world. The treatment includes radiotherapy, chemotherapy, combination chemotherapy and radiotherapy-surgery. Radiotherapy is aimed to disrupt the reproduction of malignant cells. Radiation may result in immediate and permanent complications, such as mucositis, hyposalivation, and xerostomia resulting oral hygiene becomes worse may cause imbalance of oral microba. The purpose of this study was to determine correlation between the number volume salivary colonies of Candida sp. and dose radiotherapy of head and neck cancer patients. This research is an observational cross-sectional study, subject was selected using consecutive sampling. Samples were brought to the Microbiology Laboratory of the Faculty to be cultured. Cultivation of Candida sp. by used dilution 1: 104 CFU / ml in Sabouraud dextrose agar medium and incubated for 48 hours at a temperature of 370C. After 48 hours the number of colonies were counted. The data obtained from this study were analyzed by using the Shapiro-Wilk normality, followed by Spearman correlation test. The test results showed the significant value of 0.000 (p<0,05) with r = 0.656. The conclusion is there a strong correlation between the number of colonies of Candida sp. the radiation dose received by the patient's head and neck cancer in the department of Dr. Sardjito hospital, the higher dose of radiotherapy received, the more colonies number colonies of Candida sp. in patients saliva.
