Cancer causes 7.4 million deaths in the world. Cancer is the second main causes of death, after cardiovascular. Breast cancer is the most common type of cancer that affects women. Breast cancer is the highest cause of death in women, including Indonesia. Breast cancer treatment becomes more difficult after metastasis to other tissues. Part of metastasis process such as migration and invasion is a target of cancer therapy. Jamur tiram or oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm.) contains a steroid compound called Lovastatin that is known as anticancer agent. Lovastatin has inhibitory activity of proliferation and migration in colon cancer cells and white blood cells. Lovastatin inhibits expression and secretion of MMP-9 protein in vascular smooth muscle cells, NIH 3T3 and v-H-Ras 3T3 cells. The aim of this study is to investigate cytotoxic activity, antimigration, and inhibition of expression MMP-9 protein of ethanolic extract of jamur tiram (EEJT). The cytotoxic activity was carried out using MTT assay. Scratch wound healing assay was used to determine migration inhibitory activity of EEJT while gelatine-zymography was conducted to detect MMP-9 expression toward 4T1 cells (metastatic breast cancer cells). The result showed that EEJT performed less cytotoxic activity with IC50 value of 400 µg/ml. EEJT 50 dan 100 μg/mL inhibited cells migration on 4T1 cells. In addition, EEJT decreased MMP-9 expression by 32% compared with controls . In conclusion, EEJT possess activity as antimetastasis agent by inhibition of migration and MMP-9 expression. Therefore, the further study needed be conducted to find out EEJT molecular target playing a role in metastatic pathway. Keywords : Pleurotus ostreatus, metastasis, migration, MMP-9, 4T1 metastatic breast cancer cells.
