SYNTHESIS AND DERIVATIVES KHALKON cytotoxicity assay T47D AGAINST CANCER CELLS, HeLa and WiDr FOR IN VITRO
ABSTRACT: Syntheses of chalcone derivatives (ST 1-3) and its cytotoxicity againts cancer cell lines have been investigated. The chalcones (ST 1-3) were prepared by Claisen-Schmidt condensation of 2-hydroxy-4,6-dimethoxyacetophenone with benzaldehyde, p-anisaldehyde and veratraldehyde. Synthesis of chalcones were carried out by stirring at room temperature for 48 hours using ethanol as a solvent and KOH as a catalyst. The structure of synthesized compounds were characterized using FTIR, GC-MS, 1H-NMR and 13C-NMR. The cytotoxicity of chalcone derivatives were tested againts T47D, HeLa and WiDr cancer cell lines using MTT assay. The results showed that chalcones (ST 1-3) were successfully synthesized in 84.5, 82.8 and 81.3%, respectively. The cytotoxicity test indicated that chalcone ST 1 and ST 2 had a good activity for inhibiting the growth of T47D, HeLa and WiDr cancer cells with IC50 value less than 12,5 �µg/mL. While chalcone ST 3 had a moderate activity for inhibiting the growth of HeLa cancer cells with IC50 value less than 50 �µg/mL. The chalcones (ST 1-3) had selectivity index value more than 3 that indicated good selectivity of anticancer agent.
