MicroRNA Expression BART-7-3p PTEN mRNA IN PLASMA AND BLOOD OF PATIENTS nasopharyngeal carcinoma
ABSTRACT: Nasopharyngeal Carcinoma (NPC) is an epithelial assosiated Epstein-Barr Virus (EBV) infected cancer which become an epidemic in the Asia region included Indonesia. Recently, the incidence and mortality of NPC cases increased, and there is tendency of younger ages. The poor diagnostic method for this cancer is one of the main problems for NPC treatment, thus new efficient and effective diagnostic methods is needed. MicroRNA BART-7 is one of the BART family MicroRNA that is expressed by EBV. MicroRNA BART-7 is hypothesized as one of the potential biomarker for the detection of NPC. Phosphatase and tensin homolog (PTEN) is a human tumor suppressor protein which associated in many develompents on cancer, including NPC. Mutation and deletion of PTEN gene will caused enzimatic inactivation of PTEN protein and lost of tumor suppresion ability of PTEN. MicroRNA BART-7-3p is one of the miRNA that targeted PTEN. therefore this research aim to analyze the expression of MicroRNA BART-7-3p and mRNA PTEN on NPC patient and healthy control. Analysis were performed on 64 plasma of NPC patients, 10 plasma of healthy control and 4 tissues of NPC patients by isolation of total RNA, cDNA synthesis and quantification by qPCR. The expression of MicroRNA BART-7-3p on the plasma NPC patients were higher than the healthy control. On the NPC tissues, the expression of MicroRNA BART-7-3p were very high compared to the plasma healthy control. Additionally, this research also did the measurement of mRNA PTEN which is targeted by MicroRNA BART-7-3p. The expression of mRNA on the plasma NPC patients were lower than the healthy control. Through statistical analysis it was deemed that MicroRNA BART-7-3p and mRNA PTEN has the negative correlation.
